The cysteamine film was immoboilized on a quartz crystal gold electrode’s surface by self assembly. DNA probes of lung cancer and cysteamine film were linked with carbonylic carbon nanotubes which been activated with 1 ethyl 3 (3 dimethyllaminopropyl) carbodiimide hydrochloride(EDC) and 1 hydroxy 2,5 pyrrolidinedione(NHS). Thus, a new lung cancer piezoelectric DNA sensor was constructed to detect DNA labeling lung cancer, and the sensitivity was 27.617 Hz/(μg/mL), and the linear range was from 0.02 μg/mL to 7.50 μg/mL. The DNA sensor had good regenerability, and there were not significant difference between the DNA sensor and PCR in samples detection.