The fullerene nanocapsules of liquid perfluorocarbon (PFH C60) were prepared by using the phacoemulsification extraction method first. Then each group of sample was observed the ultrasound imaging after ablation of high intensity focused ultrasound in vitro. In addition, the degassed bovine liver in vitro was directly injected into normal saline,C60 solution or PFH C60 separately before HIFU ablation. After HIFU ablation of same parameters, the gray changes of irradiation areas and coagulation necrosis in liver were observed. The results of experiment found that PFH C60 can enhance HIFU ablation and ultrasound imaging in vitro,and the PFH C60 ultrasound imaging in vitro was echoless, but after irradiated by HIFU (certain scope of parameters), it got an echo enhancement. After proceeding HIFU ablation, compared with group NS and C60,group PFH C60 showed significant differences in gray change values and squares (P＜0.01), also whose volume of coagulation necrosis became larger (P＜0.01). The research indicates that the prepared PFH C60 nanocapsules have the functions of ultrasound imaging and HIFU synergist in vitro．