The conventional acoustic localization system cannot meet the specific geometry of the array layout. An acoustic localization method based on arbitrary layout array is proposed in this paper. Since the algorithm needs to ensure that the transformation matrix is nonsingular, and at least 5 elements are required to localize the sound source, the proposed method has good engineering application prospects. The simulation of the algorithm has been carried out with MATLAB. The simulation area is 500 m×500 m. The delay standard deviation is 60 μs. The numbers of Monte Carlo are 500 times. The maximum errors of x and y coordinates are less than 0.04 m. The maximum error of zcoordinate is less than 0.1 m. It shows that this algorithm can not only effectively calculate the threedimensional positioning of the sound source, but also can be flexibly applied to detect the sound or other sound targets on the battlefield.