According to the demands of micro-manipulation tools in the field of micro-nano manipulation, a modular multifinger micro-operating tool with simplestructure, highflexibility and highprecision was designed and fabricated. Each finger in the actuator was driven by piezoelectric ceramic actuator. The experiments on the multifinger microoperating actuator under the singlefinger, doublefinger and triplefinger working modes were carried out to verify the abilities of manipulating various microtargets with sizes ranging from 10 SymbolmA@m to 800 SymbolmA@m, and the success rate of more than 85% was achieved. The pickup and release of microspheres with different sizes, textures and structures can be completed by changing the endeffectors and adjusting the operating strategy. At the same time, the experiments of the microsphere selfadhesive assembly and the microsphere operation of ultramicroscopic were carried out by using multimeans microoperation tool, which proved that the operation tool has important practical application significance.